5aa SunTag VP64 EVD

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联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: 5aa SunTag VP64 EVD 
出品公司: 爱迪基因
目录编号: 115483
存储实验室: Steven Jacobsen

载体骨架基本信息
载体名称: pMOA34
载体出品公司: --
载体抗性: 大观霉素
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 植物表达,CRISPR,合成生物学
筛选标记: 潮霉素
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS,5aa SunTag VP64 EVD
物种来源: --
插入大小: 15407 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: --
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: 5aa SunTag VP64 EVD was a gift from Steven Jacobsen (Addgene plasmid # 115483 ; http://n2t.net/addgene:115483 ; RRID:Addgene_115483)
参考文献部分: Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722

载体图谱质粒图谱和多克隆位点信息

5aa SunTag VP64 EVD质粒图谱

载体简介

载体序列

LOCUS       5aa SunTag VP64 EVD               26054 bp ds-DNA     circular SYN 21-MAR-2019
DEFINITION  CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each 
            with two guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    5aa SunTag VP64 EVD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 26054)
  AUTHORS   Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
  TITLE     Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 
            SunTag systems.
  JOURNAL   Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
  PUBMED    30760722
REFERENCE   2  (bases 1 to 26054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 21, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..26054
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4657..4712
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             4726..8829
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8833..8859
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8878..8898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8905..8925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8989..9045
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9061..9117
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9133..9189
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9205..9261
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9277..9333
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9349..9405
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9421..9477
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9493..9549
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9565..9621
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9637..9693
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     terminator      9741..10448
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(10481..10499)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10482..10499)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    10542..10566
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     oriT            11128..11237
                     /note="incP origin of transfer"
     mobile_element  11297..12064
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     CDS             12689..13837
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(15026..15043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15197..15785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15277..15296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             16882..17670
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      18345..18876
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      18353..18967
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     misc_feature    19067..19091
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     terminator      19165..19417
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(19445..20470)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(20524..20703)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_RNA        complement(21161..21236)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(21718..21793)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(22551..22601)
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     CDS             complement(22629..22790)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             complement(22821..22841)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(22866..23015)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(23055..23765)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(23095..23115)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     23710..23738
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(23856..23882)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac
       61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt
      121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc
      181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg
      241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt
      301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt
      361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg
      421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta
      481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg
      541 ttaaaagctc tgttgagcta gactcatatg catcctagga aacaagttgt aatgagttgc
      601 tggcctctct tgggattgtt catcaaagta gctgtcccta cacaccacaa cagaatggtg
      661 ttgtggagag aaagcacaga cacatccttg agatggcaag ggcacttaag tttcagagtg
      721 gtgtacctac caggttttgg ggagactgtg tcaagactgt tgtatacctg atcaatagac
      781 tccctactcc aatcttacaa ggcaaatgtc catatgaact actctatcag aaacatgcca
      841 agcttgatca cttgagagtg tttgggtgtc ttgcttttgc agcaacactg cccaaaggtg
      901 acaaacttgc acctagagcc aagcgaacca tcttcattgg ttattctgag actcaaaagg
      961 gttacaggtt gtatgacttg gataataagg tgatcatagt gagcagggat gtagttttca
     1021 gagagtttca gttccctttc aaagagtcca tctcccatga acctgatatg ttcactcgag
     1081 caggttcact tagctctgaa gatacagctt tgcagctagt gtataatgat atttttcctt
     1141 ccagctttca tactgatgat gatgatactc cagttcttat tcctgacatt gtagaggcca
     1201 atgaggatat cactttgggg actcattcta cccaaaatca tatcacttca gctgatgctg
     1261 caccttcagg ccacctgcat gctcctgcag agtcagttga gtcagctgag cctgacctag
     1321 caaatgctga atttgagcac catactgctg tagctgatcc ctctctagtt ccagcaaacc
     1381 tgcacactag acctaaacgc aatgcaggtc ctcccatctg gctcaaggac tttgtgacac
     1441 tgaacaaagg ctctagggat gttccatacc ctatagccaa ctatgtttcc tatgatcact
     1501 tgcctgttca ctatcaagct tatttgagtg ctttctccac ttatactgaa cctaaatcct
     1561 tcaaagaagc agctcaagat gagaaatgga tggaggccat gtcccttgag atacaggctc
     1621 ttgaggataa taacacctgg gagattgtcc ctttaccccc tggtaaacag cctatagggt
     1681 ccaaatgggt gtacaaaatt aaatacaaag ctaatggtga agttgacagg tttaaggcaa
     1741 ggctagtggc caagggatac actcagcaag aaggccttga ctaccatgaa actttttctc
     1801 cagtggccaa aatggtcact gtaaaagctg tcatatctgt tgctgcttcc aagggctggt
     1861 tccttttcca gatggatgtc aacaatgctt ttctacaagg tgacctcatg gaagaagttt
     1921 atatgtctct gcctcagggt tttcacagcc aaggggagac caaagtgtgc aggctgttga
     1981 atccctctat ggtttaaagc aagcatcaag gcagtggaac atcaagctta ccactgtcct
     2041 tatgcaggct ggttttatgc aaagtgctta tgatcactcc ttgtttacca aaagagaggg
     2101 gactgacctt gtcataatcc tgatttatgt ggatgatctg ttaataacag gcagcagcaa
     2161 tgttttgatt tcagaagcaa aggcaaccct gcatcagcat ttcaagatga aagatttggg
     2221 agaactaaaa tacttcttag gcattgaggt gctgagatca gaaaagggaa tcctactgaa
     2281 ccagaggaag tatgcactgg aactgatatc aggtgttggt ctgggaggct gcagaccagt
     2341 gtcaacccca atggaacaga accaaaggct gactactgta gaatatgaca agcacttagg
     2401 aaagactgat gatgcagagt tagaggatgt gggttcctat cagagactgg tgggaaagct
     2461 tctctacttg acaatcacaa ggccagatat cagctttgca gtgcaggtgc tgagtcaatt
     2521 catgcagcaa cccaaacagt cacacctgga agcagcattg agggttgtga agtacataaa
     2581 aggttctcca ggtgttagga agcggccgca tgcatatgag tctagctcaa cagagctttt
     2641 aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag aaagagattc
     2701 cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa gcataaaatc
     2761 ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac ttgacaagca
     2821 taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa tgatcttcct
     2881 ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt ctacttccag
     2941 cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc ctgtatgatc
     3001 gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc catcaaagtt
     3061 gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc gacggtgttg
     3121 tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca tctgcatctt
     3181 cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc accagctcgt
     3241 ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca ttcttcagat
     3301 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag
     3361 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc
     3421 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa
     3481 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc
     3541 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc
     3601 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag
     3661 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
     3721 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
     3781 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
     3841 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
     3901 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa
     3961 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
     4021 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
     4081 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
     4141 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtattttct tcttcttctt
     4201 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
     4261 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
     4321 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
     4381 ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc
     4441 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
     4501 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
     4561 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagggatca tcaaaaagtt
     4621 tgtacaaaaa agcaggctct ttaaagtatt tttacaacaa ttaccaacaa caacaaacaa
     4681 caaacaacat tacaattact atttacaatt acaaaaaaag ttaacatgga caagaagtac
     4741 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     4801 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     4861 aacctgatcg gcgccctgct gttcgacagc ggagaaacag ccgaggccac ccggctgaag
     4921 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     4981 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     5041 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     5101 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     5161 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     5221 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     5281 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     5341 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     5401 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat tgccctgagc
     5461 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     5521 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     5581 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     5641 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     5701 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     5761 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta catcgatggc
     5821 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     5881 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     5941 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     6001 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     6061 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     6121 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     6181 gccagcgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     6241 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtacaa cgagctgacc
     6301 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     6361 aaagccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     6421 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     6481 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     6541 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     6601 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     6661 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     6721 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     6781 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     6841 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     6901 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     6961 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     7021 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     7081 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     7141 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     7201 caggaactgg acatcaaccg gctgtccgac tacgatgtgg acgctatcgt gcctcagagc
     7261 tttctgaagg acgactccat cgataacaaa gtgctgactc ggagcgacaa gaaccggggc
     7321 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcgccag
     7381 ctgctgaatg ccaagctgat tacccagagg aagttcgaca atctgaccaa ggccgagaga
     7441 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     7501 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     7561 aacgacaaac tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     7621 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     7681 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     7741 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     7801 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     7861 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     7921 aacggcgaaa caggcgagat cgtgtgggat aagggccggg actttgccac cgtgcggaaa
     7981 gtgctgtcta tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     8041 agcaaagagt ctatcctgcc caagaggaac agcgacaagc tgatcgccag aaagaaggac
     8101 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     8161 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     8221 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     8281 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     8341 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     8401 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     8461 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caaacactac
     8521 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     8581 aatctggaca aggtgctgag cgcctacaac aagcacagag acaagcctat cagagagcag
     8641 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     8701 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     8761 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     8821 ggaggcgacg cctatcccta tgacgtgccc gattatgcca gcctgggcag cggctccccc
     8881 aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga aagtggacgg cattggtagt
     8941 gggagcaacg gcagcagcgg atccaacggt ccgactgacg ccgcggaaga agaacttttg
     9001 agcaagaatt atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga
     9061 gaagaactgc tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg
     9121 agcggaagtg gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga
     9181 ctaaagaaag ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac
     9241 gaagtggctc ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac
     9301 cacctcgaaa atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta
     9361 tccaagaatt atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga
     9421 gaggaactgc tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga
     9481 tcgggcagtg gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga
     9541 ttaaagaaag gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat
     9601 gaggtagcga gactaaaaaa ggggagtggt tctggcgagg aattgctatc gaaaaattat
     9661 catcttgaga acgaagttgc taggctcaaa aagggctcag gctcaggcta acccgggggc
     9721 gcgccatgat aactagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga
     9781 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
     9841 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
     9901 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
     9961 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
    10021 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
    10081 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
    10141 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
    10201 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
    10261 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
    10321 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
    10381 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg
    10441 ctcagcagga ccggcatgca agctagctta ctagtgatat tctatagtgt cacctaaatc
    10501 tgcggccgcg gtaccactag tacgcgtagg cctgagctct ttgacaggat atattggcgg
    10561 gtaaacgata tcggcacctt cgaccgcctg ttccgcagag gtgcaggcct cgatctgaaa
    10621 cccgaaccgc tggagattgc gggagcagcg agcagtagcc tcggggtcga tgtcgtaaag
    10681 tcgtatccga tcgacgccga tcagcgcctt gaaggccaaa gcctggaact cactttgggc
    10741 accgttgccg atcagcgcca tcgtgcgcga atctttacgg gccagatact ttgccgcgat
    10801 cgcggaggtc gcggccgttc gcaaggccgt caggattgtc atttccgaca gcagcagcgg
    10861 atagccgcta tcgacatcgg agagcacgcc gaacgcggtt accggcctct tcatcgggaa
    10921 tgcgcgcgac cttcagcatc gccggcatgt ccccctggcg gacgggaagt atccagctcg
    10981 acctcgacgg atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
    11041 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
    11101 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
    11161 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
    11221 tctgcgaggc tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga
    11281 aaccaagcca accaggggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
    11341 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
    11401 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta
    11461 aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca
    11521 ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc
    11581 tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca
    11641 gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc
    11701 taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc
    11761 gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc
    11821 ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg
    11881 aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct
    11941 gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct
    12001 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
    12061 tacccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga
    12121 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc
    12181 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc
    12241 atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg
    12301 cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag
    12361 caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt
    12421 ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc
    12481 catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc ggaataccaa
    12541 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
    12601 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
    12661 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
    12721 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
    12781 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
    12841 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
    12901 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac
    12961 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
    13021 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
    13081 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
    13141 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
    13201 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
    13261 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
    13321 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
    13381 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
    13441 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
    13501 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
    13561 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
    13621 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
    13681 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
    13741 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
    13801 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
    13861 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg
    13921 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac
    13981 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac
    14041 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg
    14101 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat
    14161 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag
    14221 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa
    14281 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc
    14341 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc
    14401 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc
    14461 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct
    14521 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc
    14581 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc
    14641 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc
    14701 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca
    14761 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg
    14821 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg
    14881 cgactcgaac ctacagttgt ttccttactg ggctttctca gccgggatgg cgctaagaag
    14941 ctattgccgc cgatcttcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    15001 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    15061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    15121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    15181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    15241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    15301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    15361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    15421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    15481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    15541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    15601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    15661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    15721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    15781 atcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    15841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    15901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    15961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    16021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    16081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    16141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    16201 ttaaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtgcc aaaagccgcg
    16261 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
    16321 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
    16381 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
    16441 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
    16501 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
    16561 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
    16621 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
    16681 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
    16741 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
    16801 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
    16861 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
    16921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
    16981 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
    17041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
    17101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
    17161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
    17221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
    17281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
    17341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
    17401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
    17461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
    17521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
    17581 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
    17641 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
    17701 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg
    17761 aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca
    17821 attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt
    17881 tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac
    17941 aactctgaga gcaaatacga taatagttca tccaattacg acaatagtcg caacggaaat
    18001 cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc
    18061 aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa
    18121 ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata
    18181 aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac
    18241 tagcctgctc tctaataaaa tgttaggagc ttggctgcca tttttggggt gaggccgttc
    18301 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    18361 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
    18421 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
    18481 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
    18541 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
    18601 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
    18661 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
    18721 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
    18781 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
    18841 caagttttcc gcgaggtatc cacaacgccg gcggccggcc gcggtgtctc gcacacggct
    18901 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg
    18961 caaccagccc ggtgagcgtc ggaaagggtc gaggaattcc gcctaaccgg ctcagttctg
    19021 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggcggc aggatatatt
    19081 caattgtaaa tggcttcatg tccgggaaat ctacatggat cagcaatgag tatgatggtc
    19141 ccatcgataa gcttggcaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt
    19201 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
    19261 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
    19321 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
    19381 atcttaagaa actttattgc caaatgtttg aacgatcggg gatcgatccc ggtcggcatc
    19441 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
    19501 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
    19561 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
    19621 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
    19681 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
    19741 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
    19801 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
    19861 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
    19921 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
    19981 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
    20041 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
    20101 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
    20161 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
    20221 tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
    20281 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
    20341 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
    20401 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
    20461 gctttttcat atcttattgc ccccctagag tcgagatctg gattgagagt gaatatgaga
    20521 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat
    20581 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt
    20641 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg
    20701 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
    20761 actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa
    20821 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
    20881 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
    20941 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
    21001 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
    21061 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagcttgca tgcctgcagg
    21121 tcgactctag acacggggtg gtttggttta aacaaaaaaa gcaccgactc ggtgccactt
    21181 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaaccata
    21241 ttctttcatg gtatcacaat cactacttcg actctagctg tatataaact cagcttcgtt
    21301 ttcttatcta agcgatgtgg gacttttgaa gattgttttc aacttaaatg ggcctatata
    21361 agaaatacta ttgttctttc ccatataaat gggcctgctt ctcttctttc agattcccag
    21421 gggccttttg aagattatct tcatatctta agaatgaaga tgttttattc aatcaaattc
    21481 ttgaaggttc gatgcctaat cattctaatc ctgggacaaa ctatgaaaca agatacaaaa
    21541 actccgaatg gaaagttaaa aagaagaaaa cgaaagctac ggttcaagaa aatgtaagct
    21601 gataaacaaa aaaaaactgt atgaacgaag aagaagaaaa aaagctaaga agaaatgatg
    21661 tattgtgcgg aaggcaagtc gagtttccgt tgttcaacga aagcttgttt aaaaaaagca
    21721 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt
    21781 ctagctctaa aacacagata atagctttat tcacaatcac tacttcgact ctagctgtat
    21841 ataaactcag cttcgttttc ttatctaagc gatgtgggac ttttgaagat tgttttcaac
    21901 ttaaatgggc ctatataaga aatactattg ttctttccca tataaatggg cctgcttctc
    21961 ttctttcaga ttcccagggg ccttttgaag attatcttca tatcttaaga atgaagatgt
    22021 tttattcaat caaattcttg aaggttcgat gcctaatcat tctaatcctg ggacaaacta
    22081 tgaaacaaga tacaaaaact ccgaatggaa agttaaaaag aagaaaacga aagctacggt
    22141 tcaagaaaat gtaagctgat aaacaaaaaa aaactgtatg aacgaagaag aagaaaaaaa
    22201 gctaagaaga aatgatgtat tgtgcggaag gcaagtcgag tttccgttgt tcaacgaaag
    22261 ctttcctaaa cgtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
    22321 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
    22381 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
    22441 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
    22501 ccaaatgttt gaacgatcgg ggaaattcga gctcaaactt acggccatgg cgtaggcggc
    22561 cttttccgtt gtgatcttcg cggtctcctc cttgtttttg gactagtacc accaccgcta
    22621 ccaccacctt cggttaccgt gaaggttttg gtagcgtcgt cgtaggtcca ttcaccgtca
    22681 acaccgttgt cgttagcgta ctgtttgaaa actttttccg cggtagcagc gtcaacagct
    22741 tcggtggtgg tttcaccttt cagggtttta ccgttcagga taagcttgta ctcttcggtc
    22801 cgagatcctc ctcccgtacg gacctttctc ttcttctttg gagcccctcc gccaccgcca
    22861 ctacctagca tatctagatc aaagtcatct agggcatcgg agcccaacat atctaaatcg
    22921 aagtcgtcca atgcatctga gcctaacatg tctaggtcga agtcgtctaa cgcgtctgaa
    22981 ccaagcatat ctaagtcaaa atcgtctaaa gcatcccctc cgccaccgcc cgtacgaccg
    23041 gtccgacctc cacctttgta gagctcatcc atgccatgtg taatcccagc agcagttaca
    23101 aactcaagaa ggaccatgtg gtcacgcttt tcgttgggat ctttcgaaag gacagattgt
    23161 gtcgacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
    23221 tgataatggt ctgctagttg aacggaacca tcttcaacgt tgtggcgaat tttgaagtta
    23281 gctttgattc cattcttttg tttgtctgcc gtgatgtata cattgtgtga gttaaagttg
    23341 tactcgagtt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ctttaactcg
    23401 atacgattaa caagggtatc accttcaaac ttgacttcag cacgcgtctt gtaggtcccg
    23461 tcatctttga aagatatagt gcgttcctgt acataacctt cgggcatggc actcttgaaa
    23521 aagtcatgcc gtttcatgtg atccggataa cgggaaaagc attgaacacc ataggtcaga
    23581 gtagtgacaa gtgttggcca cggaacaggt agttttccag tagtgcaaat aaatttaagg
    23641 gtgagttttc cgtttgtagc atcaccttca ccctctccac ggacagaaaa tttgtgccca
    23701 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
    23761 ttgctaccgc ttccacctcc acctggatcc aagcttccgc cgcctcctga accaccaccg
    23821 ccactacctc ctcccccaga acctccgcct ccaccagcgt aatctggaac atcgtatggg
    23881 tagctgctca cggtcaccag ggtgccctgg ccccagtagt cgaacaggcc ggtcacgcag
    23941 tagtacaggg cggtgtcgtc gctgcgcacc ttgctcatct gcaggtacac ggtgttcttg
    24001 ccgttgtcct tgctgatgat gaagcggtcc ttcagggcgc tgttgtagtc ggtgatgccg
    24061 tcgccccaga tcacgccgat ccactccagg ccgcggccgg gggcctggcg cacccagttc
    24121 acgccgtagt cggtcaggct gaagccgctc acggcgcagc tcagcttcag gctgccgccg
    24181 ggctgcacca ggccgccgcc gctctccagc agcttcacct cgctgccgcc gccgctgctg
    24241 ccgccgccgc cgctgccgcc gccgccgctg ccgccgccgc cgcgcttcag ctccaccttg
    24301 gtgccctggc cgaacaccca gtggttgctg taccacaggg cgcagaagta ggtggcgaag
    24361 tcctcgggct gcaggctgct gatggtcagg gtggccttgt cgccgatcag gctgccgctg
    24421 aagcggctgg gcacgccggg ggcgcggttg ttggtgccgc cgatcaggcc cttgaacagc
    24481 ttgccgggct tctcctgcac ccagctggcg tagttgctgg tggtcacggc gccggtgctg
    24541 ctgcggcagg tgatggtcac gcggtcgccc acgctggcgc tcaggctgct ggggctctgg
    24601 gtcatcacga tgtcggggcc cataaaccct aggtgttaat cagaaaaact cagattaatc
    24661 gacaaattcg atcgcacaaa ctagaaacta acaccagatc tagatagaaa tcacaaatcg
    24721 aagagtaatt attcgacaaa actcaaatta tttgaacaaa tcggatgata tctatgaaac
    24781 cctaatcgag aattaagatg atatctaacg atcaaaccca gaaaatcgtc ttcgatctaa
    24841 gattaacaga atctaaacca aagaacatat acgaaattgg gatcgaacga aaacaaaatc
    24901 gaagattttg agagaataag gaacacagaa atttaccttg atcacggtag agagaattga
    24961 gagaaagttt ttaagatttt gagaaattga aatctgaatt gtgaagaaga agagctcttt
    25021 gggtattgtt ttatagaaga agaagaagaa aagacgagga cgactaggtc acgagaaagc
    25081 taaggcggtg aagcaatagc taataataaa atgacacgtg tattgagcgt tgtttacacg
    25141 caaagttgtt tttggctaat tgccttattt ttaggttgag gaaaagtatt tgtgctttga
    25201 gttgataaac acgactcgtg tgtgccggct gcaaccactt tgacgccgtt tattactgac
    25261 tcgtcgacaa ccacaatttc taacggtcgt cataagatcc agccgttgag atttaacgat
    25321 cgttacgatt tatatttttt tagcattatc gttttatttt ttaaatatac ggtggagctg
    25381 aaaattggca ataattgaac cgtgggtccc actgcattga agcgtatttc gtattttcta
    25441 gaattcttcg tgctttattt cttttccttt ttgttttttt ttgccattta tctaatgcaa
    25501 gtgggcttat aaaatcagtg aatttcttgg aaaagtaact tctttatcgt ataacatatt
    25561 gtgaaattat ccatttcttt taatttttta gtgttattgg atatttttgt atgattattg
    25621 atttgcatag gataatgact tttgtatcaa gttggtgaac aagtctcgtt aaaaaaggca
    25681 agtggtttgg tgactcgatt tattcttgtt atttaattca tatatcaatg gatcttattt
    25741 ggggcctggt ccatatttaa cactcgtgtt cagtccaatg accaataata ttttttcatt
    25801 aataacaatg taacaagaat gatacacaaa acattctttg aataagttcg ctatgaagaa
    25861 gggaacttat ccggtcctag atcatcagtt catacaaacc tccatagagt tcaacatctt
    25921 aaacaagaat atcctgatct gaagaatgtg gaggctttag tcccttggat acttgggagg
    25981 ctgtggaaga acagaaacga gctggtgctt aaagggaggg aatttggaac caatgaggta
    26041 ttagtaagga caca
//

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